ABSTRACT
Objective To investigate the value of serological screening combined with fetal aneuploidy prenatal noninvasive DNA test ( NIPT) in prenatal diagnosis ,and provide guidance for reducing the birth of children with genetic defects in the future .Methods A retrospective analysis was conducted in 15282 pregnant women with prenatal counseling who performed serological screening and NIPT test .The high risk and critical TANG recommended NIPT test and severe abnormal karyotype children recommend termination of pregnancy .Results Down syndrome screening results showed that 804 cases of 15,282 cases of serological screening samples were detected in high risk , the high risk rate was 5.26％.A total of 804 patients with high risk of Don screen were further tested with noninvasive DNA,which was positive in 10 cases.Among them,8 cases were confirmed by amniocentesis ,including 5 cases of trisomy 21,1 case of trisomy 18 and 2 cases of sex chromosome abnormality (45,XO in one case and 47,XYY in one case),the consistency was 100.00％.Conclusion Noninvasive gene detection of fetal aneuploidy has the advantages of noninvasive ,safe and accurate .It has a wide range of clinical value in the diagnosis of fetal chromosomal abnormalities .
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Objective To evaluate the consistence in the detection of antibodies against HIV-1 between a new rapid test using oral mucosal transudate (OMT) samples and ELISA using serum samples. Methods Two-hundred patients who were positive for anti-HIV-1 antibodies by serum ELISA and confirmed by Western blot to be infected with HIV, and 600 healthy human controls negative for anti-HIV-1 antibodies by serum ELISA, were eligible for this study. OMT samples were collected from these subjects and subjected to a rapid test for anti-HIV-1 antibodies. The factors influencing the performance of the rapid test were analyzed. Results Of the 200 OMT specimens from HIV-infected patients, 198 showed positive reaction, 2 showed negative reaction. Among the 198 positive reactions, 192 (96%) were "clear" and easy to make decisions, 4 (2%) were "faint", 2(1%) were "very faint" and required professionals to make decisions. The rapid test was negative in all the 600 OMT specimens from the control group. Conclusions The consistence in the detection of anti-HIV-1 antibodies between the OMT rapid test and serum ELISA was 99% in HIV-positive specimens, 100% in HIV-negative specimens, and 99.75% in all the specimens.
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<p><b>OBJECTIVE</b>To develop a rapid assay for simultaneous detection of HIV p24 antigen (Ag) and anti-HIV antibody (Ab).</p><p><b>METHODS</b>HIV-1 gp41 antigen and HIV-2 gp36 antigen were expressed by recombinant baculovirus insect system and purified by immunochromatography. p24 monoclonal antibody (mAb) was obtained from p24 hybridoma cell line. Purified antigen and mAb were dot blotted to nitrocellular membrane; 20 nm colloidal gold-anti-human IgG ab and p24 ab complex were used for this test. Previously detected 39 sera specimens were tested in this study to compare with the result of HIV test with commercial HIV test kit.</p><p><b>RESULTS</b>20 mg/L purified gp41 Ag and gp36 Ag were obtained from recombinant baculovirus-insect cell system; 1.5 mg/L p24 mAb was obtained from p24 mAb hybridoma cell line. Compared the test result of 39 sera with commercial HIV test kits, consistency rate was 100%.</p><p><b>CONCLUSIONS</b>The rapid assay for simultaneous detection of HIV p24 antigen and anti-HIV antibody provides a simple, sensitive and reliable test for HIV diagnosis.</p>
Subject(s)
Humans , AIDS Serodiagnosis , Gene Products, env , HIV Antibodies , Blood , HIV Antigens , HIV Core Protein p24 , Blood , HIV Envelope Protein gp41 , HIV Infections , Diagnosis , HIV-1 , Allergy and Immunology , HIV-2 , Allergy and Immunology , Reagent Kits, Diagnostic , Reference Standards , env Gene Products, Human Immunodeficiency VirusABSTRACT
Objective To investigate Chlamydia trachomatis infection and gene variants in child- bearing women in Beijing. Methods Endocervical samples were collected from 177 patients in a family planning clinic and a gynecologic clinic. C.trachomatis infection was screened with a fast diagnostic kit .Major outer membrane protein(MOMP) gene was amplified by a nested PCR . A 1.1kb amplified product was obtained and studied by restriction fragment length polymorphism analysis(RFLP). Variant domains of MOMP gene VS1,VS2,VS3,VS4 were sequenced with 373A automated sequencing system. Results C. trachomatis was found in 9 of 177 samples. Four samples were identified as F genotype, 3 E genotype and 2 D genotype. Variant domains of MOMP gene, VS1, VS2, VS3, VS4 were sequenced and the results were consistent with those of RFLP, however, mutations were found in MOMP gene in 4 samples. Conclusion C.trachomatis urogenital infection has been identified in a portion of child- bearing women in Beijing. C.trachomatis can be well genotyped with RFLP or gene sequencing of MOMP gene.
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Effect of China-made isocrystal pirenzepine ( IPRZ ) on experimental ulcer produced by reserpine in rats was studied & the antiulcer effect of China-made IPRZ was compared with that of China-made PRZ. Our results showed that China-made IPRZ given by either sc ( 25-100 mg/kg ) or ig ( 25-100 mg/kg ) exerted a marked antiulcer effect on reserpine-induced gastric ulcer in rats.The antiulcer effect was increased with the increase of dose of China-made IPRZ. When China-made IPRZ was given by sc, its antiulcer effect was as potent as China-made PRZ, but when it was given by ig, its antiulcer effect was lower than China-made PRZ .This result suggest that the bioavailabili-ty of China-made IPRZ given by ig was lower than China-made PRZ.
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The pharmacokinetics of thyrotropirrreleasing hormone (TRH) made in China was studied in rabbits. TRH concentrations in plasma, urine and various tissues were measured by RIA. After intravenous injection of TRH, the plasma concentration-time curve was shown to fit a two compartment open model with the following pharmacokinetic parameters: 0.8 mg/kg, T1/2?=0.551 min, T1/2? = 9.374 mm, VI = 3.018 ml/kg, Vb = 22.659 ml/kg and AUC = 0.479g. L-1. m-1; 2.0mg/kg, T1/2? = 0.629 mm, T1/2?= 9.734 min, VI = 5.135 ml/kg, Vt = 28.848 ml/kg, and AUC = 0.962g. L-1. m-1; 5.0mg/kg, T1/2? =0.720 min, T1/2?= 10.738 min, VI = 6.120 ml/kg, Vb = 31.928 ml/kg and AUC=2.540 g. L-1. m-1. TRH was distributed widely in tissues and concentrated mainly in the kidney and pituitary. TRH was eliminated partly by the kidney and 21.9% was excreted within 2h after intravenous injection.